KMID : 1098420220300050311
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Korean Journal of Medicinal Crop Science 2022 Volume.30 No. 5 p.311 ~ p.318
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Effects of Gastrodia elata Blume on Apoptotic Cell Death of Liver Cancer Cells by Expression of Bcl-2, Bax, and AMPK¥á
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Park Jae-Hyun
Kang Min-Ho Hong Ji-Woo Kim So-Hee Hwang Youn-Seon Park Jae-Hoon Kim Jin-Woo
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Abstract
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Background: This study was carried out to investigate the anticancer effects of Gastrodia elata Blume extract (GEE) in human hepatoma-derived Hep3B cells by apoptotic cell death.
Methods and Results: To evaluate the anticancer effect, a Hep3B cell viability assay and a realtime quantitative reverse transcription polymerase chain reaction (qRT-PCR) on GEE were performed, which was obtained extracted by ultrasound-assisted extraction and then used to treat the Hep3B cells. Cell viability was inhibited to 48.2% (4.0 §·/§¢). To identify anticancer mechanisms using qRT-PCR, mRNA expressions of B-cell lymphoma-2, its associated Bax protein, and 5¡¯- AMP-activated protein kinase were examined with a comparative ¡âCT method. A qualitative analysis of the bioactive compounds in GEE was coducted using liquid chromatography tandem mass spectrometry, gastrodin and phloretin were identified as the two major compounds.
Conclusions: These findings demonstrate that GEE can decrease liver cancer cell viability and control apoptosis gene expression to have anticancer effects, GEE could be employed as a natural anticancer agent with less side effects than those of traditional anticancer medications.
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KEYWORD
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astrodia elata Blume, Anticancer, Cell Viability, Gene Expression, Hep3B, Realtime Quantitative Reverse Transcription Polymerase Chain Reaction
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